Phase-transition based transduction in a biosensor


J. F. Bardeau, A. S. Eberhardt, R. M. Nyquist, A. N. Parikh, A. P. Shreve, B. I. Swanson, Synthetic Metals 102, 1452 (1999)

Shifts in the main phase transition temperatures caused by a highly specific, protein-receptor interaction in a hybrid bilayer architecture are probed utilizing temperature programmed infrared vibrational spectroscopy. The specific case of cholera toxin attachment to glycolipid receptors embedded in a phospholipid outer layer at a hydrophobized silicon surface is investigated. Potential applications of the approach as a signal amplification method in biosensors will be discussed.