Cholesterol-enriched domain formation induced by viral-encoded, membrane active amphipathic peptide


Joshua M. Hanson*, Douglas L. Gettel*, Seyed R. Tabaei, Joshua Jackman, Min Chul Kim, Darryl Y. Sasaki, Jay T. Groves, Bo Liedberg, Nam-Joon Cho, Atul N. Parikh (*, Equal First Authors), Biophysical Journal 110, 176-187, 2016


The α-helical (AH) domain of the hepatitis C virus nonstructural protein NS5A, anchored at the cytoplasmic leaflet of the endoplasmic reticulum, plays a role in viral replication. However, the peptides derived from this domain also exhibit remarkably broad-spectrum virocidal activity, raising questions about their modes of membrane association. Here, using giant lipid vesicles, we show that the AH peptide discriminates between membrane compositions. In cholesterol-containing membranes, peptide binding induces microdomain formation. By contrast, cholesterol-depleted membranes undergo global softening at elevated peptide concentrations. Furthermore, in mixed populations, the presence of ∼100 nm vesicles of viral dimensions suppresses these peptide-induced perturbations in giant unilamellar vesicles, suggesting size-dependent membrane association. These synergistic composition- and size-dependent interactions explain, in part, how the AH domain might on the one hand segregate molecules needed for viral assembly and on the other hand furnish peptides that exhibit broad-spectrum virocidal activity.

DOI: 10.1016/j.bpj.2015.11.032J